Fig. 3. Analysis of plasmid DNA from B.burgdorferi transformants. Left panel: an ethidium bromide-stained field inversion gel of plasmid DNA from untransformed B31-A (lane 1) or from B31-A transformed with pKK81 (lane 2; minus the lp17 telomere), pGCL10-2 (lane 3; plus the 140 bp replicated telomere) and pGCL13–1 (lane 4; with a 140 bp replicated mock telomere). The migration positions of 48.5 and 23.1 kb markers and wild-type lp17 are shown. Center panel: a Southern blot of the gel in the left panel, hybridized with Probe 1, corresponding to bp 414–1231 from the left end of lp17 (Figure 2). Right panel: a Southern blot of the gel in the left panel, hybridized with Probe 2 from the kan gene. The migration positions of intact lp17 integrants (I), resolved integrants (R) and wild-type lp17 (Wt) are shown on the right side of the figure.