Skip to main content
. 2005 Aug;7(8):788–798. doi: 10.1593/neo.05106

Figure 6.

Figure 6

Figure 6

Figure 6

Effects of blocking EGFR on HER-2 activation. (A) MDA-361 cells (containing HER-2 and not EGFR), MDA-468 cells (containing EGFR and not HER-2), and MCF-10HER2/E6E7 cells were treated with DMSO or 0.1, 0.5, or 1.0 µM Iressa. HER-2 and EGFR were immunoprecipitated, separated on 7.5% SDS-PAGE, blotted, and probed for PTyr. At a concentration of 0.5 µM, Iressa had no effect on HER-2 activation, whereas that concentration abrogated EGFR activation. (B) Cells were treated with DMSO or 0.5 µM Iressa for 48 or 72 hours. HER-2 and EGFR were immunoprecipitated, separated on 7.5% SDS-PAGE, blotted, and probed for PTyr. Nonspecific bands are once again presented to show an equivalent loading of immunoprecipitates from each experiment. (C) Cells were plated and a sample was counted at day 1. Another sample was treated with 0.5 µM Iressa or DMSO for 5 days and counted.