Figure 1.

Schematic diagram of full-length nonmuscle myosin II-A and II-B heavy chains and the truncated NMHC II-A, ΔN592, and ΔC170 constructs. The full-length and truncated NMHC II constructs are all fused to GFP and under the control of the tetracycline-responsive promoter, so that they are only expressed in the absence of doxycycline (Dox) and expression of the transgenes will be turned off by the addition of Dox. Stable cell lines were established for each of the constructs except ΔC170. ATP- and actin-binding domains are indicated. Numbers indicate amino acid residues.