Cell rounding induced by the expression of
GFP-ΔN592, but not the full-length myosin heavy chain (NMHC II-A) in
HeLa Tet-Off cells. Cells were cultured for 3 d without Dox (a, c,
e, and g) or with Dox (b, d, f, and h). The images were taken from live
cells by using an Axivert 35 microscope (Carl Zeiss). Note the presence
of the GFP signal only in a and e (−Dox) and the rounded morphology of
cells expressing GFP-ΔN592 (e and g) in contrast to cells expressing
GFP-NMHC II-A (a and c). The original magnification was 320×.