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. 2000 Oct;11(10):3617–3627. doi: 10.1091/mbc.11.10.3617

Figure 8.

Figure 8

GFP-ΔN592 does not localize to the cytokinetic ring. GFP-NMHC II-A is distributed throughout the cytoplasm during prophase and metaphase (a and b) and localized to the cleavage furrow during early telophase (c) and the midbody during late telophase (d). ΔN592 shows a similar distribution to full-length myosin II-A during prophase and metaphase, although forming more aggregates (e–h). Note, however, that, unlike full-length NMHC II-A, it does not localize to the cleavage furrow during early telophase (g) or the midbody during late telophase (h). Transfection of HeLa cells with GFP-NMHC II-B, which is not normally expressed in these cells, shows that similar to full-length GFP-NMHC II-A, and in contrast to ΔN592, it localized to the cleavage furrow during early telophase (k) and the midbody during late telophase (i). Tubulin is detected by a rhodamine-labeled secondary antibody (red). Bar, 5 μm.