Skip to main content
. 2005 Sep;7(9):873–882. doi: 10.1593/neo.05256

Figure 1.

Figure 1

Figure 1

Estrogen induces BRCA-1 promoter activity in transiently transfected MCF-7 cells. (A) MCF-7 cells were precultured for 4 days in phenol red-free DMEM containing 5% charcoal dextran-stripped FBS (Hyclone Laboratories, Logan, UT). Then, a 1.69-kb fragment of the BRCA-1 5′ flanking region driving the expression of a luciferase cassette (pGL3BRCA-1) was transiently transfected, using Lipofectamine Plus (Life Technologies, Inc.), into MCF-7 cells, which were cultured in DMEM or DMEM plus 10 nM E2 (Sigma, St. Louis, MO) for various periods of time. (B) The treatment with E2 induces promoter activity from a positive control vector (p3XERE) transfected into MCF-7 cells. (C) Western blot analysis with antibodies for BRCA-1 (Ab-2) and β-actin (Ab-1) (Oncogene Research Products) documents that BRCA-1 protein levels are induced in MCF-7 cells cultured for 24 hours in DMEM plus 10 nM E2. (D) HeLa and (E) HCT116 cancer cells were cotransfected with pGL3BRCA-1 and either a plasmid encoding for ERα (pERα) or an empty vector (pCR3.1). Transfected cells were cultured in DMEM or DMEM plus 10 nM E2 for 24 hours. Control plates were transfected with p3XERE. Bars represent mean luciferase units corrected for the internal control renilla ±SE from two independent experiments performed in quadruplicate.