Figure 6.

Sulf-2 expression in normal mouse tissues and mammary gland tumors. Detergent lysates of the indicated tissues were subjected to immunoprecipitation and Western blot analysis with a Sulf-2 (H2.1) antibody or normal rabbit IgG, as described in Materials and Methods section. (A) Analysis of expression in MMTV-Neu tumor, MMTV-Wnt1 tumor, and cell extracts of COS-7 cells transfected with MSulf-2. (B) Four sets of normal mammary gland from FVB/N mouse, and hyperplastic and tumor tissues from a littermate MMTV-Wnt1 transgenic mouse were subjected to immunoprecipitation/Western blot analysis, as described above. Sulf-2 was detected in all of the tumors, in two of four of the hyperplastic tissues, and in none of the normal tissue extracts.