Table 1.

Objectives

Magnification	Field of View	Working Distance	Immersion	Description
×4	3.25 mm	13 mm	No	UPLFL
×10	1.3 mm	10 mm	No	UPLFL
×10	1.3 mm	3.1 mm	No	UPLAPO
×20	0.65 mm	1.6 mm	No	UPLFL
×40	0.325 mm	0.51 mm	Water	UPLFL
×20 SS	200 μm	100 μm	Water	MLS-D1W100W
×30 SS A	260 μm	50 μm	Water	MLS-D3W050W
×30 SS B	260 μm	200 μm	Water	MLS-D3W200W
×6 LS	670 μm	200 μm	No	IV-OB13F67W20
×20 LS	200 μm	200 μm	Water	IV-OB13F20W20
×27.3 LS	220 μm	200 μm	Water	IV-OB35F22W20

All stick objectives were custom-developed for the current study. SS, short stick objective; LS, long stick objective.