Table 1.
Primer Pairs Used in RT-PCR
| Gene | Forward Primer | Reverse Primer | Product (bp) |
|---|---|---|---|
| Gata-4 | GCCCAAGAACCTGAATAAATCTAAG | AGACATCGCACTGACTGAGAACGTC | 208 |
| Gata-6 | TTCCCCCACAACACAACCTACAG | GTAGAGCCCATCTTGACCCGAATAC | 118 |
| AFP | GGTGTAGCGCTGCAAACGATG | AATTTAAACTCCCAAAGCAGCACGA | 210 |
| STAT3 | GGGTGGAGAAGGACATCAGCGGTAA | GCCGACAATACTTTCCGAATGC | 198 |
| RUNX1 | CTCAGGTTTGTCGGTCGAAGTGGAA | CCGCAGCTGCTCCAGTTCAC | 216 |
| RUNX2 | CTCCCTGAACTCTGCACCAAGTCCT | GGGGTGGTAGAGTGGATGGACG | 156 |
| RUNX3 | CCGAGCCATCAAGGTGACCGTGGAC | GGGCTGGCTGCTGAAGTGGCTTGT | 187 |
| Osteocalcin | CCCTCACACTCCTCGCCCTATT | AAGCCGATGTGGTCAGCCAACTCGT | 259 |
| ALP | CACTGCGGACCATTCCCACGTCTT | GCGCCTGGTAGTTGTTGTGAGCATA | 206 |
| IBSP | GGGCAGTAGTGACTCATCCGAAGAA | CTCTCCATAGCCCAGTGTTGTAGCA | 166 |
| Nanog | GCTGAGATGCCTCACACGGAG | TCTGTTTCTTGACTGGGACCTTGTC | 163 |
| Oct 3/4 | TGGAGAAGGAGAAGCTGGAGCAAAA | GGCAGATGGTCGTTTGGCTGAATA | 186 |
| β-III Tubulin | CTGCTCGCAGCTGGAGTGAG | CATAAATACTGCAGGAGGGC | 141 |
| β-Actin | GCGGGAAATCGTGCGTGACATT | GATGGAGTTGAAGGTAGTTTCGTG | 229 |