Skip to main content
. 2005 Oct;7(10):921–929. doi: 10.1593/neo.05361

Figure 1.

Figure 1

Figure 1

Growth inhibition of glioma cells by rapamycin and EKI-785. U87 and U251 cells were incubated for 72 hours with the indicated concentrations of rapamycin and EKI-785, both individually and in 1:100 molar ratio combinations. The effects on cell number were assessed using an MTS colorimetric cell growth assay. (A) Changes in absorbance, relative to a value of 1 for untreated controls, are shown for the indicated drug concentrations and combinations. Results have been plotted as mean ± SEM for three independent experiments. *P < .05 and #P = .06, as indicated by Student's t test results, for rapamycin versus EKI-785 + rapamycin. †P < .05, for EKI-785 vs EKI-785 + rapamycin. (B) The MTS data from (A) were analyzed for synergy using the median effect method, and the CI values were plotted relative to corresponding fractional affects. Each point on the graph is labeled with a letter that corresponds to a rapamycin/EKI-785 combination noted with the same letter in panel A. CI values less than 1 reflect a synergistic interaction between the drugs.