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Monitoring the response of Hepa-1 spheroids to TOOKAD-based PDT by DW-MRI. Hepa-1 spheroids were MRI-scanned before and 20 hours post-PDT. Diffusion maps of untreated controls (A) and PDT-treated spheroids (B) were generated. PI stainings of untreated controls (C) and PDT-treated spheroids (D) are shown. Anti-HNE stainings of untreated control (E) and PDT-treated (F) spheroid are shown. NC, necrotic core; VR, viable rim; FVR, former viable rim; SP, spheroid.
