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. 2000 Oct;11(10):3645–3660. doi: 10.1091/mbc.11.10.3645

Figure 3.

Figure 3

Chromosomal locations of Wpkci and chPKCI demonstrated by FISH with the mixed probes. (A) A metaphase set from the female chicken embryonic fibroblast was hybridized with biotinylated pGP-3 (for Wpkci), DIG-labeled BAC clone 224D8 (for chPKCI), a 1:1 mixture of biotinylated and DIG-labeled pUGD1201 (for the EcoRI family on the W chromosome), and a 1:1 mixture of biotinylated and DIG-labeled pCZTH5–8 (for the pFN-1 macrosatellite on the Z chromosome). The biotinylated probes were detected with FITC–avidin, and the DIG-labeled probes were detected with sheep anti-DIG Fab fragment followed by rhodamine-labeled anti-sheep Fab. Locations of Wpkci on the nonheterochromatic end of the W chromosome and chPKCI near the centromere on the long arm of the Z chromosome are illustrated in B. The chPKCI locus on the Z chromosome was assigned by measuring 122 FISH figures.