Figure 8.

Determination of the molar ratio of mRNAs for Wpkci and chPKCI. Different amounts of the linearized cDNA clone, pWpkci-8 or pchPKCI-3 (A), or different amounts of poly(A)⁺ RNA from gonads plus mesonephroi of 6-d (stage 29) female embryos or 6-d female whole embryos (B) were electrophoresed, blotted onto nylon membranes, and hybridized together with ³²P-labeled cDNA probe specific for Wpkci, chPKCI, or GAPDH, respectively, and subjected to fluorescence image analysis. The ratio of signal intensity in A was obtained by comparing slopes of signal intensity for each cDNA clone. In B, the signal intensity for Wpkci mRNA or chPKCI mRNA was first corrected with that for GAPDH mRNA (the intensity of Wpkci mRNA was multiplied by 1.003 for the sample from whole embryo and by 1.38 for the sample from gonads plus mesonephroi), the slope of signal intensity for Wpkci mRNA was corrected by the ratio obtained in A, and the molar ratio of mRNAs for Wpkci and chPKCI was calculated.