Figure 9.

Whole mount in situ hybridization to the part of a 4.5-d (stage 25) female or male chicken embryo containing mesonephroi and undifferentiated left (arrowhead) and right (arrow) gonads, with DIG-labeled antisense or sense riboprobe for Wpkci (A) or chPKCI (B). Wpkci transcripts were detected only in the female embryo, and the chPKCI transcripts were detected in both female and male embryos with the antisense probe, followed by the reaction with anti-DIG antibody–coupled alkaline phosphatase. Bar, 1 mm.