Fig. 3. Blocking of the COPI transport enhanced the co-localization of TCRα and ERGIC53 in COPI mutant cells. LdlF CHO cells were transiently transfected with TCRα and myc-tagged ERGIC53 at 34°C. After incubation at a non-permissive temperature (39.5°C) for 6 h, the cells were fixed and evaluated by confocal laser scan microscopy with double labeling for TCRα with a rabbit anti-murine TCRα antiserum and for ERGIC53 with an anti-myc mAb (9E10) (A). Scale bar represents 10 µm. The pixel intensity (arbitrary units) in the fluorescence of TCRα (red) or ERGIC53 (green) was recorded by confocal laser scan microscopy along the line from the bottom to the top in the lower panel (B). The peak intensities of TCRα and ERGIC53 were co-localized well at a non-permissive temperature (39.5°C).