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. 2003 Jan;5(1):53–62. doi: 10.1016/s1476-5586(03)80017-9

Figure 1.

Figure 1

Validation of the cDNA array CGH technique. Genomic DNA from the neuroblastoma cell line IMR32 was used in both conventional CGH on metaphase chromosomes (inset, A) and cDNA array CGH (B). In the ideogram profiles generated for the cDNA array CGH results, the normalized fluorescence intensity ratios from individual replicates (n=4) are given in yellow, and their average is plotted in red. Note the profile for chromosome Y is not shown due to the low representation of genes from this chromosome on the microarray. Chromosome CGH of IMR32 showed expected MYCN amplification at 2p24, with an additional amplification at 2p15 (A). These changes were also observed by cDNA array CGH, but at higher resolution (B). cDNA array CGH was able to determine that the gene amplification at 2p15 corresponded to MEIS1, which was recently reported using other methods.