Effect of resveratrol on UVB-induced activation of NF-κB: EMSA. The normal human keratinocytes were treated with resveratrol (5, 10, and 25 µM for 24 hours for dose-dependent studies or 5 µM for 12, 24, and 48 hours for time-dependent studies) after which the cells were washed and exposed to UVB radiation in PBS. Six hours following UVB exposure, the nuclear lysates were prepared. The EMSA was performed as detailed under Materials and Methods. C1, C2 and C3 represent controls; biotin-EBNA control DNA, biotin-EBNA control DNA+EBNA extract, biotin-EBNA control DNA+EBNA extract +200-fold molar excess of unlabeled EBNA DNA, respectively. The data shown here are from representative experiment repeated three times with similar results.