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. 2003 Jan;5(1):74–82. doi: 10.1016/s1476-5586(03)80019-2

Figure 4.

Figure 4

Effect of resveratrol on UVB-induced activation of NF-κB/p65: ELISA. The normal human keratinocytes were treated with resveratrol (5, 10, and 25 µM for 24 hours for dose-dependent studies or 5 µM for 12, 24, and 48 hours for time-dependent studies) after which the cells were washed and exposed to UVB radiation in PBS. Six hours following UVB exposure, the nuclear lysates were prepared for ELISA. The effect on NF-κB/p65 was evaluated using ELISA as detailed in Materials and Methods. The data are expressed as mean±SEM from four experiments, conducted at least in triplicate.