Figure 5.

Effect of resveratrol on UVB-induced degradation and phosphorylation of IκBα. The normal human keratinocytes were treated with resveratrol (5, 10, and 25 µM for 24 hours for dose-dependent studies or 5 µM for 12, 24, and 48 hours for time-dependent studies) after which the cells were washed and exposed to UVB radiation in PBS. Six hours following UVB exposure, the postnuclear lysates (cytosol) were prepared. The immunoblot analysis was performed for IκBα or phospho-IκBα using appropriate antibodies as described under Materials and Methods. For every immunoblot, equal loading of protein was confirmed by stripping the blot and reprobing with β-actin antibody (data not shown). The data shown here are from representative experiment repeated four times with similar results.