Skip to main content
. 2003 Jan;5(1):63–73. doi: 10.1016/s1476-5586(03)80018-0

Figure 3.

Figure 3

Effects of estrogen and progesterone on WISP-2 mRNA expression in a MCF-7 cell line. (A, C, and E) Exponentially growing MCF-7 ER+ breast cancer cells in phenol-red-free DMEM containing 10% FBS were serum starved for 3 days before exposure to 17β-E2 (1x10-8 M) or P4 (1x10-8 M) or in combination for different times. The total RNA was extracted from cells harvested at indicated times and analyzed by Northern blotting using nonradioactive DIG-labeled PCR generated probes for WISP-2 and GAPDH. (B, D, and F) The arbitrary values indicate WISP-2 mRNA concentrations. Data displayed as mean±SD from three separate experiments. P value was determined by Student's t test. *P <.01 versus control; **P < .005 versus control; ***P <.0001 versus control.