Figure 4.

Dose-dependent effects of estrogen and progesterone on WISP-2 mRNA expression in MCF-7 cells. (A and C) Exponentially growing MCF-7 ER⁺ breast cancer cells in phenol-red-free DMEM containing 10% FBS were serum starved for 3 days before exposure to different doses of 17β-E₂ and P₄ for 24 and 1 h, respectively. Total RNA was extracted and analyzed by RNA blotting using nonradioactive DIG-labeled PCR generated probes for WISP-2 and GAPDH. (B and D) The arbitrary values indicate WISP-2 mRNA concentrations. Data displayed as mean±SD from three separate experiments. P value was determined by Student's t test. *P <.001 versus control; **P<.01 versus control.