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. 2003 Jan;5(1):63–73. doi: 10.1016/s1476-5586(03)80018-0

Figure 6.

Figure 6

Effects of estrogen on ER-α-transfected and non-transfected normal human mammary epithelial cells. (A) Serum-starved ER-transfected or (C) only vector-transfected cells were exposed to 1x10-8 M 17β-estradiol for 2 or 24 h. Total RNA was extracted and analyzed by RNA blotting using nonradioactive DIG-labeled PCR generated probes for WISP-2 and GAPDH. (B and D) The arbitrary values indicate WISP-2 mRNA concentrations. Data displayed as mean±SD from three separate experiments. P value was determined by Student's ttest. *P <.001 versus control; **P <.004 versus control.