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Tumor-specific protective immunity in long-term survivor mice. IL-21 + IL-2 treated mice that survived disease free for 150 days were rechallenged with B16F10 (5 × 105) or 3LL(5 × 104) tumor cells. All 3LL challenged mice (4/4) exhibited mean tumor growth to ≥ 200 mm2 by day 32; all B16F10 challenged mice (5/5) were protected. Naïve mice challenged with the same B16F10 (5/5) or 3LL (5/5) tumor cells had rapid mean tumor growth to ≥ 200 mm2 by day 25.
