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. 2006 Jul 18;4(8):e232. doi: 10.1371/journal.pbio.0040232

Figure 7. Scatchard Analysis of Vitamin D3 Using Smo-Expressing P. pastoris .

Figure 7

(A) 24 h methanol induction (buffered methanol-complex medium; BMMY) of Smo-transformed P. pastoris yielded successful Smo expression as shown by Western blot analysis with an antibody directed against Smo.

(B) Scatchard analysis of heterologous competition with cyclopamine representing the binding of [3H]-D3 to Smo-expressing (solid circles, MeOH induced) and nonexpressing (open circles, noninduced) yeast strains. The solid line indicates the induced high-affinity binding, whereas the dotted line indicates nonspecific low-affinity binding. Scatchard analysis and fitting was performed as described earlier [ 53].