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. Author manuscript; available in PMC: 2006 Jul 13.
Published in final edited form as: Nature. 2005 Jul 21;436(7049):406–409. doi: 10.1038/nature03713

Figure 2.

Figure 2

Rv2869c and its proteolytic activity are required for mycobacterial cording
  1. Single colonies of the indicated BCG strains at 5x magnification. Scale bar indicates 2 mm.
  2. Auramine-Rhodamine stained smears of the indicated M. tuberculosis strains examined by fluorescence microscopy at 400 x magnification.
  3. Conserved HExxH and FDG motifs of Rv2869c are required for complementation of the mutant cording phenotype. The M. bovis BCG Rv2869c null mutant was complemented either with the wild type Rv2869c, Rv2869c(H21A), or Rv2869c(D340A) and single colonies were assessed for return of wild type colony morphology.