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. 2006 Jul 18;3(8):e253. doi: 10.1371/journal.pmed.0030253

Figure 1. Lipoprotein Particles of Infected Humans Contain Schistosomal Surface Proteins.

Figure 1

(A) Lipoprotein particles of control and patient sera were separated from soluble proteins by KBr density gradient centrifugation. Proteins from gradient fractions were analysed by SDS-PAGE and Western blotting against schistosome-specific glycoproteins (sGP), schistosomal GPI-anchored protein SM200, and human apolipoprotein B (ApoB). Note the presence of sGP and SM200 in the top fractions that contain the lipoprotein particles. Strong background staining is due to high protein concentrations in serum; data are representative of six different patient sera.

(B) Control (C) and patient (P1 and P2) sera were mock-treated (−) or treated with PI-PLC (+) and subjected to immunoprecipitation with biotinylated antibodies against either sGP (left) or SM200 (right). Immunocomplexes were isolated with streptavidin-agarose beads and analysed by SDS-PAGE and Western blotting for ApoB. Streptavidin beads alone did not pull down ApoB in patient sera (unpublished data). Antibodies were raised against a peptide sequence or a common glycoconjugate and recognize the protein irrespective of the presence of the diacylglycerol moiety.