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. 2006 Jul 18;3(8):e253. doi: 10.1371/journal.pmed.0030253

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© 2006 Sprong et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Adult S. mansoni worms were incubated overnight in medium with [³⁵S]-amino acids and [¹⁴C]-palmitic acid in the presence of normal (full) or delipidated (DL) FCS, and media were precleared by high-speed centrifugation.

(A) Media were subjected to KBr density gradient centrifugation, and fractions were analysed for schistosomal proteins by SDS-PAGE followed by autoradiography (top) and for schistosomal lipids by TLC followed by autoradiography (bottom). The top and second top fractions of the gradients are shown. Note the presence of prominent protein bands at 25, 70, and 250 kDa in the top fraction of full but not of delipidated medium.

(B) Top fractions from KBr density gradient centrifugations of mock-treated (− PI-PLC) or PI-PLC treated (+ PI-PLC) normal media (full) or delipidated media (DL) were analysed for schistosome-specific glycoproteins (top) and for SM200 (middle) by Western blotting and for schistosomal lipids by thin-layer chromatography followed by autoradiography (bottom).