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CHO-LDLA cells lacking (−) or retransfected with (+) LDL receptor were incubated with control (C) or patient (P1 and P2) sera. Cell lysates were subjected to SDS-PAGE and Western blotting for endocytosed SM200 or schistosome-specific glycoproteins. As loading controls, the medium was probed for ApoB, and the cells for calreticulin and for the presence of transfected LDL-receptor.
