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. 2006 Jul 18;3(8):e253. doi: 10.1371/journal.pmed.0030253

Figure 6. Lipoproteins from Infected Humans Are Decorated with Antibodies and Can Be Endocytosed Via the FcγRIIa Receptor.

Figure 6

(A) Control (C) and patient (P1 and P2) sera were incubated with protein A-Sepharose beads. Bound material was eluted and subjected to SDS-PAGE and Western blotting for ApoB.

(B) Lipoprotein particles were isolated from control and patient sera, stained for endogenous antibodies present on lipoprotein particles using protein A-gold, and subjected to electron microscopy.

(C) CHO-LDLA cells lacking (−) or transfected with (+) FCγRIIa receptor were incubated with control (C) or patient (P1 and P2) sera. Cell lysates were subjected to SDS-PAGE and Western blotting for endocytosed ApoB (ApoB [Cells]). A proteolytic fragment [56] of ApoB was detected at ~80 kDa. As load controls, the medium was probed for ApoB (ApoB [Medium]), and the cells for calreticulin.