Figure 10.

Reconstitution of ganglioside biosynthesis and expression in B6A4C1 cells by activated Cdc42. Confocal fluorescence images (greyscale) show representative IgE-sensitized RBL-2H3 cells or B6A4C1 cells infected for 12 h with the indicated vaccinia virus constructs. Cells were labeled with FITC-cholera toxin B (A-E), then fixed and postlabeled with Cy3-DNP-BSA (F-J) as described in Methods. Some fluorescence bleedthrough of FITC into the Cy3 channel is noted. Scale bar = 10 μm for all images.