Figure 7.

Arachidonic acid production (A) and PLD activation (B) in stimulated RBL-2H3 and B6A4C1 cells. Cells were grown in the presence of ³H-arachidonic acid (A) or ³H-myristic acid (B) as described in Methods. IgE-sensitized cells were incubated with buffer (open bars), 50 ng/ml DNP-BSA (filled bars), or 500 nM A23187 plus 50 nM PMA (hatched bars) for 45 min at 37°C in the absence (A) or presence (B) of 0.5% (vol/vol) ethanol. Liquid scintillation counting was used to quantitate radiolabeled arachidonic acid metabolites released into the supernatant (A) or phosphatidylethanol isolated by TLC (B).