Figure 8.

Polymerization of actin in RBL-2H3 and B6A4C1 cells using different stimulants. Cells were sensitized with IgE and incubated with buffer (open bars), 50 ng/ml DNP-BSA, 5 min (diagonally hatched bars), 10 nM PMA, 5 min (solid bars), or 10 μM NECA, 1 min (horizontally hatched bars). Total F-actin content of the cells was assayed by fixing the cells with formaldehyde, permeabilizing with detergent, and measuring the amount of bound NBD-phallacidin. The data are expressed as the F-actin ratio which is calculated as (F-actin in activated cells - background)/(F-actin in unstimulated cells - background). The experimental error in all cases is less than 8%.