Figure 2.

Representative study of the artificial recruitment assay. Two strains of yeast, MaV103 and CG1945, which differ in promoter context for the HIS3 reporter gene, were transformed with each of the indicated DB–TAF fusion constructs and assayed for the ability to grow on AT containing media. Cell growth on AT (40 mM for MaV103 or 3 mM for CG1945) is indicative of activated expression of HIS3 reporter gene. Yeast growth in each panel is a serial dilution of slashes from a single colony. All strains grew robustly on control plates of synthetic complete media lacking leucine and tryptophan (not shown).