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. 2003 Feb 15;31(4):1364–1373. doi: 10.1093/nar/gkg205

Figure 2.

Figure 2

Endonuclease digestion of supercoiled pBR322 plasmid DNA and analysis of proteins associated with open circular intermediate. (A) Supercoiled pBR322 plasmid DNA (1 µg) was incubated at 37°C in the presence of the active 48–64 kDa endonuclease preparation (0.01 mg/ml) from Figure 1B. Aliquots (100 ng) were removed at the time points indicated and analyzed by agarose gel electrophoresis. Control lane indicates plasmid DNA incubated in the absence of protein for 60 min. (B) SDS–PAGE analysis of proteins associated with open circular plasmid DNA separated in (A).