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EndoG activity associated with inner mitochondrial membrane detected by degradation of 403 bp fragment of mtDNA. Mitoplasts (0.25 mg/ml) were incubated with amplified D-loop fragment (100 ng) for the times indicated at 25°C. Lane 1, molecular weight markers; lanes 2–4, time course using sucrose-washed mitoplasts; lanes 5–7, time course using salt-washed mitoplasts.
