Figure 5.

Effect of a MPT upon the retention of endoG activity and cytochrome c protein by rat liver mitochondria. (A) Mitochondria (75 µg/ml) were suspended in 2 mM HEPES, pH 7.5, 0.25 M sucrose, 10 mM succinate and 1 mM potassium phosphate at 25°C, in the presence or absence of cyclosporin A (CyA, 4.5 µg/ml), and a MPT induced by the addition of Ca²⁺ (1 µM final concentration). Absorbance was monitored at 540 nm. (B and D) EndoG activity released and retained by the mitochondria upon induction of a MPT. (C and E) Cytochrome c protein released and retained by the mitochondria upon induction of a MPT. Lane 1, control mitochondria; lane 2, Ca²⁺-induced MPT; lane 3, Ca²⁺-induced MPT following pre-treatment (5 min) with CyA.