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. 2003 Feb 15;31(4):1282–1291. doi: 10.1093/nar/gkg206

Figure 3.

Figure 3

Order of addition experiments with E2dbd and polyamides. (A) An EMSA was performed in which the radiolabeled HPV E2 BS4 probe was preincubated with polyamide (or the vehicle DMSO) under equilibrium conditions prior to the addition of E2dbd as described in Materials and Methods. DNA was incubated in the absence of either polyamide (lane 1) or E2dbd (lanes 2–6) or preincubated with DMSO (lane 2) or 0.13 (lane 3), 0. 64 (lane 4), 3.2 (lane 5) or 16 nM PA1 (lane 6) prior to the addition of a fixed concentration of the DNA binding domain of E2 (10 nM). (B) An EMSA was performed in parallel in which the radiolabeled HPV E2 BS4 probe was preincubated with the DNA binding domain of E2 under equilibrium conditions prior to the addition of 10 nM polyamide PA1 (or the vehicle DMSO) as described in Materials and Methods. DNA was preincubated with E2dbd (lanes 1–5) prior to the addition of a variable concentration of DMSO (lane 1) or 0.13 (lane 2), 0. 64 (lane 3), 3.2 (lane 4) or 16 nM PA1 (lane 5).