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. 2006 Jun 30;103(28):10636–10641. doi: 10.1073/pnas.0604194103

Fig. 4.

Fig. 4.

Ectopic expression of HIMP1-a in MIN6 and βTC3 β cells protects cells from apoptosis and extends cell survival under hypoxia (5% O2) for 24 h. (A-C) Analysis in stable MIN6HIMP1-a clonal cells. (A) Immunoblot, 50 μg of protein per lane. (B) Confocal immunofluorescent staining in MIN6HIMP1-a clone 10 cells. (Scale bar, 50 μm.) (C) Protective effects of HIMP1-a on MIN6 cell survival at hypoxia for 24 h. (D and E) Transient transfection assay in βTC3 cell. (D) Images of triple staining of TUNEL (red), HIMP1-a (green), and DAPI (blue) in βTC3 cells transfected with either vector (a and b) or HIMP1-a (c and d) under hypoxic conditions for 24 h. (Scale bar, 50 μm.) (E) Protective effects of HIMP1-a on βTC3 cell survival at hypoxia for 24 h.