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. 2006 Jun 30;103(28):10636–10641. doi: 10.1073/pnas.0604194103

Fig. 5.

Fig. 5.

Ectopic expression of HIMP1-a in MIN6 and βTC3 β cells protects cells from apoptosis and extends β cell survival at high (25 mM) or low (2.5 mM) glucose levels after exposure for 3 days. (A–C) Analysis in stable MIN6HIMP1-a clonal cells. (A) Images of DIC and double staining of TUNEL (red) and DAPI (blue) in MIN6HIMP1-a clone 10 and control cells at low and high glucose. (Scale bar, 50 μm.) (B) Protective effects of HIMP1-a on MIN6 cell survival at low glucose. (C) Protective effects of HIMP1-a on MIN6 cell survival at high glucose. (D–G) Transient transfection assay in βTC3 cells. (D) Immunoblot analysis of HIMP1-a expression in βTC3 cells. (E) An image sample of triple staining of TUNEL (red image of B), HIMP1-a (green image of B), and DAPI (blue image of A) in βTC3 cells transfected with HIMP1-a for 3 days at high glucose. (Scale bar, 50 μm.) (F) Protective effects of HIMP1-a on βTC3 cell survival at low glucose. (G) Protective effects of HIMP1-a on MIN6 cell survival at high glucose.