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Verification of expression profiles. Downstream analysis of two developmentally regulated transcripts - (a) and (b) are the two transcripts. Candidate cDNAs were selected on the basis of the original DDRT-PCR profile (I), purified from co-migrating sequences by mSSCP (II) and used as probes in northern analysis (III). To correct for variation in RNA loading, northern blot membranes were re-probed for expression of the mouse beta actin gene (IV).
