Table 1.

Oligonucleotide sequences of primers used

Anchored primer

dT12AA 5'-d(TTTTTTTTTTTTAA)-3'

dT12GA 5'-d(TTTTTTTTTTTTGA)-3'

dT12CA 5'-d(TTTTTTTTTTTTCA)-3'

dT12AG 5'-d(TTTTTTTTTTTTAG)-3'

dT12GG 5'-d(TTTTTTTTTTTTGG)-3'

dT12CG 5'-d(TTTTTTTTTTTTCG)-3'

dT12AC 5'-d(TTTTTTTTTTTTAC)-3'

dT12GC 5'-d(TTTTTTTTTTTTGC)-3'

dT12CC 5'-d(TTTTTTTTTTTTCC)-3'

dT12AT 5'-d(TTTTTTTTTTTTAT)-3'

dT12GT 5'-d(TTTTTTTTTTTTGT)-3'

dT12CT 5'-d(TTTTTTTTTTTTCT)-3'

Random primer

(arbitrary but defined sequence)

P3 5'-d(GCCGTTCCAT)-3'

R1 5'-d(GGAACTCCGT)-3'

R2 5'-d(GGCAAGTCAC)-3'

R4 5'-d(AGGACCGCTA)-3'

R5 5'-d(CGGACCCCGG)-3'

R7 5'-d(TACAACGAGG)-3'

R8 5'-d(TGGATTGGTC)-3'

R9 5'-d(TGGTAAAGGG)-3'

R10 5'-d(TCGGTCATAG)-3'

R11 5'-d(TACCTAAGCG)-3'

R12 5'-d(CTGCTTGATG)-3'

R14 5'-d(GATCGCATTG)-3'

R18 5'-d(GGAACCAATC)-3'

M2 5'-d(CACAGTTTGC)-3'

M3 5'-d(CCACAGAGTA)-3'

S 5'-d(GCGACCCATG)-3'

A1 5'-d(ACAGAGCACA)-3'

A2 5'-d(ACGTATCCAG)-3'

Oligo(dT) primer mix

(poly(A) anchored)

MA Equal mix of dT12AA, dT12GA and dT12CA

MG Equal mix of dT12AG, dT12GG and dT12CG

MC Equal mix of dT12AC, dT12GC and dT12CC

MT Equal mix of dT12AT, dT12GT and dT12CT

Extended random primer

5'-d(GTCAGAATTC-random primer)-3'

Extended oligo(dT) primer

5'-d(GTCAGAATTCT12)-3'