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. 2006 Jul 3;103(28):10793–10798. doi: 10.1073/pnas.0602493103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 3. — Muscle-specific dPink1 RNAi results in myopathology and age-dependent apoptosis in the IFMs. (A–D) EM analysis of IFM ultrastructure of flies with the following genotypes: Mhc-Gal4/+ (A), Mhc-Gal4>UAS-dPink1 RNAi (B), Mhc-Gal4>UAS-dPink1 RNAi; UAS-hPink1 (C), and Mhc-Gal4>UAS-dPink1 RNAi; UAS- hPink1ΔC (D). Arrows, swollen mitochondria; arrowheads, rod body-like deposits. (E and F) EM analysis of TTM ultrastructure in Mhc-Gal4/+ (E) and Mhc-Gal4>UAS-dPink1 RNAi (F) flies. Scale bars (5 μm) are shown at the lower left corner of each image. (G and H) TUNEL staining of thoracic musculatures from newly eclosed (G) and 1-week-old (H) Mhc-gal4>dPink1 RNAi flies. Left, DAPI staining; Center, TUNEL staining; Right, merged images. Arrows point to TUNEL-positive nuclei.