Figure 6.

Inhibition of cell growth and cell cycle progression induced by 1-Chl and its parent compounds in 293 cells. (A) Growth inhibition of 293 cells treated for 96 h with 0.001–5 µM of 1-Chl (shaded circles), Chl (open squares) or polyamide 1 (shaded squares). The percentage inhibition of cell growth was determined using standard sulphorhodamine method as described in Materials and Methods. The data points represent the average of at least two independent experiments done in triplicate. (B) Drug-induced cell cycle perturbation in 293 cells. Cellular DNA content in cells treated for 18 h with 0.01, 0.1 and 1 µM of 1-Chl (left panel) or Chl (right panel), and then post-incubated in fresh medium for 24 h was evaluated by flow cytometry as described in Materials and Methods.