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. 2003 Feb 15;31(4):1180–1190. doi: 10.1093/nar/gkg217

Figure 2.

Figure 2

EKLF is expressed at high levels in transfected cells and stable minichromosome cell lines. (A) Fluorescence microscopy of K562 cells transfected with pEGFP. At 36 h after transfection up to 80% of cells expressed GFP under the conditions employed. (B) Western blot of protein extracts (30 µg) from K562 cells transiently transfected with pSG5EKLF and from K562 cell lines stably expressing EKLF from minichromosomes. After PAGE and transfer to a nylon membrane, the blot was probed with anti-EKLF polyclonal antibodies and proteins detected by ECL. Lane 1, K562 cells transfected with pSG5 empty vector; lane 2, K562 cells transfected with pSG5EKLF; lane 3, high-expressing K562 cell clone carrying an EKLF minichromosome; lane 4, low-expressing K562 cell clone carrying an EKLF minichromosome; lane 5, MEL cell extract; lane 6, K562 cell extract; lane 7, recombinant 6-His tagged EKLF (D.A.Jackson and A.Dean, unpublished results). Mr, molecular weight markers.