Table 2. Recombination frequencies.
Recombination substrate | na | Conversion frequency (×104)b | Fold increasec | |
---|---|---|---|---|
Glucose | Galactose | |||
P × C: HOinc-2L | 4/4 | 0 | 716 ± 320 | >700 |
P × C: HOinc-2R | 3/3 | 0 | 101 ± 27 | >100 |
P × C: HOinc-LMR | 3/3 | 0 | 87 ± 53 | >87 |
Direct repeat: HOinc-2L | 10/10 | 0.3 ± 0.8 | 959 ± 389 | 3200 |
Allelic: HOinc-2L | 5/25 | 0 | 305 ± 141 | >300 |
aNumber of determinations for glucose-/galactose-grown cells.
bAverage conversion frequencies (±SD) were calculated as the number of recombinant products per colony on medium lacking tryptophan (P × C) or leucine (direct repeat), or on YPD (allelic) for glucose- or galactose-grown cells (GALHO repressed or induced). For each determination 300 to 5000 colonies were scored.
cRatio of conversion frequencies for induced:repressed cultures.