Table 2. Suppression of an amber codon in gusA mRNA mediated by three novel amber suppressor tRNAs in transformed Arabidopsis calli.
Relative GUS activity (%)a | |
---|---|
pIG121/GUS amber | 0.01 ± 0.00 |
pIG121/GUS amber + pBI/AtM1-amU70 (tRNAMet suppressor) | 0.09 ± 0.02 |
pIG121/GUS amber + pBI/AtY3II-amiG7 (tRNATyr suppressor) | 0.61 ± 0.03 |
pIG121/GUS amber + pBI/NtS2-am (tRNASer suppressor) | 10.45 ± 1.47 |
pIG121/GUS wt | 100.00 ± 6.92 |
aActivities are the means of three experiments. ± Standard error.