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Competition assay for the ability of Cy5.-annexin V conjugates to bind PS on the surface of camptothecin-treated Jurkat T cells. Displacement of FITC-annexin V (FACSFL1 channel) with a 10-fold excess of Cy5.5-annexin V preparations labeled with increasing numbers of Cy5.5 dyes per mole of protein. As n decreases (back to front), Cy5.5-annexin Vs become more effective inhibitors of FITC-annexin V binding.
