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. 2003 Feb 15;31(4):1191–1196. doi: 10.1093/nar/gkg223

Figure 3.

Figure 3

Figure 3

Trapping assay of various amounts of purified MutM, Nei and Nth duplex oligonucleotides containing 5hmU:A or 5hm:G mispairs. The 5hmU:A or 5hmU:G base-pair (Oligo 1/Oligo 3 and Oligo 1/Oligo 4, respectively)-containing duplex oligonucleotides (50 fmol) were incubated at 37°C for 30 min without (lanes 1, 7 and 13) or with purified proteins (MutM, lanes 2–6; Nei, lanes 8–12; Nth, lanes 14–18). The products were separated by electrophoresis on 15% polyacrylamide gels. MutM (lane 1, 0 pmol; lane 2, 0.44 pmol; lane 3, 0.88 pmol; lane 4, 1.8 pmol; lane 5, 3.5 pmol; lane 6, 7.0 pmol); Nei (lane 7, 0 pmol; lane 8, 0.42 pmol; lane 9, 0.84 pmol; lane 10, 1.7 pmol; lane 11, 3.4 pmol, lane 12, 6.7 pmol); Nth (lane 13, 0 pmol; lane 14, 0.38 pmol; lane 15, 0.76 pmol; lane 16, 1.5 pmol; lane 17, 3.1 pmol; lane 18, 6.1 pmol).