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. 2003 May;5(3):255–266. doi: 10.1016/S1476-5586(03)80057-X

Figure 4.

Figure 4

Figure 4

(A) Morphologic alterations of LNCaP cells following HMBME treatment. LNCaP cells were treated with either DMSO or with different concentrations of HMBME (5, 25, and 50 µM) for 24 hours. Photomicrographs of these cells were taken by phase contrast microscopy using a Nikon Microscope with a digital camera system Coolpix 995 at a magnification of 20 x (Nikon, Tokyo, Japan). The picture shown here shows cells treated with 25 µM HMBME for 24 hours. Arrows indicate apoptotic cells. (B and C) Induction of apoptosis following treatment with HMBME. Cells were treated with HMBME (25 µM for 24 hours) and induction of apoptosis was detected by FITC-Annexin staining through flow cytometry and acridine/orange staining. Both the adherent and floating cells were collected by trypsinization for quantification of apoptosis as described in Materials and Methods section. A representative graph of FITC-Annexin is shown (B). Data shown for acridine orange/ethidium bromide staining are average ± SD of two independent experiments conducted in triplicate. Cells were counted in four different fields for each sample (panel C).