Figure 1.

Binding of RAFL antibodies to mouse VEGFR2 in ELISA. The extracellular domain of mouse VEGFR2 was immobilized on plastic by incubating 96-well plates with 1 µg/ml purified protein. RAFL antibodies were added at concentrations ranging from 6.7 to 0.005 nM. The bound antibodies were detected with goat antirat IgG conjugated to peroxidase. Results show absorbance at 490 nm that reflects peroxidase activity. Each point is the average of triplicate measurements, the standard deviations of which did not exceed 5% of the mean value.